Tyrosine hydroxylase involved in cuticle tanning and reproduction in the 28‐spotted potato ladybeetle, Henosepilachna vigintioctopunctata

Shimin Chen; Satyabrata Nanda; Mujuan GuoLan KongChunxiao YangZhuoqi LiuRan GaoBaoli QiuYoujun ZhangXuguo ZhouHuipeng Pan

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Tyrosine hydroxylase involved in cuticle tanning and reproduction in the 28‐spotted potato ladybeetle, Henosepilachna vigintioctopunctata

机译：Tyrosine hydroxylase involved in cuticle tanning and reproduction in the 28‐spotted potato ladybeetle, Henosepilachna vigintioctopunctata

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Abstract BACKGROUND Tyrosine hydroxylase (TH), a melanin synthesis pathway enzyme hydroxylating tyrosine into 3,4‐dihydroxyphenylalanine, is involved in the pigmentation and sclerotization of insect cuticles. However, the role of TH in 28‐spotted potato ladybeetle (Henosepilachna vigintioctopunctata), an emerging pest of the solanaceous crops has been explored to a limited extent. In this study, we integrated dietary RNA interference (RNAi) and hematoxylin and eosin (H&E) staining with various bioassays to analyze the role of tyrosine hydroxylase (HvTH) throughout the developmental processes of Henosepilachna vigintioctopunctata. RESULTS The results revealed that ingestion of dsHvTH led to cuticle tanning impairment, arrested larval feeding in the first and second instars of Henosepilachna vigintioctopunctata, and subsequently resulted in 100% mortality. The H&E staining assays revealed that dsHvTH prevented new abdominal cuticle formation. A pharmacological study using 3‐iodo‐tyrosine (3‐IT), a HvTH inhibitor, disrupted larval–larval–pupal cuticle tanning during the third–fourth instar larval development and eventually failed to pupate. Similarly, dsHvTH fed to fourth instars hindered larval–pupal–adult cuticle tanning, and the eclose adults were 100% malformed. Ingestion of dsHvTH or 3‐IT significantly down‐regulated HvTH, HvDDC, Hvebony, and Hvlaccase2 expression and reduced dopamine levels. Finally, HvTH silencing in adult females substantially reduced the offspring hatching rates. CONCLUSIONS The collective results of the study suggested that HvTH plays conserved roles in larval–pupal–adult cuticle melanization and sclerotization while exhibiting a novel function in Henosepilachna vigintioctopunctata reproduction. ? 2022 Society of Chemical Industry.

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《Pest Management Science》 |2022年第9期|3859-3870|共12页
作者
Shimin Chen; Satyabrata Nanda; Mujuan GuoLan KongChunxiao YangZhuoqi LiuRan GaoBaoli QiuYoujun ZhangXuguo ZhouHuipeng Pan;
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South China Agricultural University;

Centurion University of Technology and Management;

Eastern Kentucky UniversityChinese Academy of Agricultural SciencesUniversity of Kentucky;

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原文格式 PDF
正文语种英语
中图分类植物保护;
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Henosepilachna vigintioctopunctata; tyrosine hydroxylase; cuticle tanning; feeding behavior; dopamine;

Tyrosine hydroxylase involved in cuticle tanning and reproduction in the 28‐spotted potato ladybeetle, Henosepilachna vigintioctopunctata

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