Is the Medium Still the Message? Culture-Independent Diagnosis of Gastrointestinal Infections

Sood Neil; Carbell Gary; Greenwald Holly S.Friedenberg Frank K.

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Is the Medium Still the Message? Culture-Independent Diagnosis of Gastrointestinal Infections

机译：Is the Medium Still the Message? Culture-Independent Diagnosis of Gastrointestinal Infections

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Infectious diarrhea is caused by a variety of pathogens, including viruses, bacteria, and parasitic organisms. Though the causative agent of diarrhea has historically been evaluated via stool cultures, recently, culture-independent diagnostic tests (CIDT) have been developed and utilized with increasing frequency. Current practice guidelines recommend their use as adjuncts to stool cultures for diagnosing acute and chronic diarrhea. The three principal CIDT are microscopy, enzyme-based immunoassays (EIAs), and molecular based polymerase chain reaction (PCR). This review explores the common causes of infectious diarrhea, the basics of stool culture, the diagnostic utility of these three culture-independent modalities, and the strengths and weaknesses of all currently available clinical techniques. It also outlines considerations for specific populations including returning travelers and those with inflammatory bowel disease.

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来源
《Digestive Diseases and Sciences》 |2022年第1期|16-25|共10页
作者
Sood Neil; Carbell Gary; Greenwald Holly S.Friedenberg Frank K.;
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作者单位

Lewis Katz Sch Med,Temple Univ;

Sect Gastroenterol & Hepatol,Temple Univ;

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原文格式 PDF
正文语种英语
中图分类消化系及腹部疾病;
关键词
Diarrhea; Polymerase chain reaction (PCR); Microbial Culture; Culture-independent diagnostic test (CIDT); Enzyme-linked immunosorbent assays (ELISA); Enzyme immunoassay (EIA); Clinical Diagnostics; CLOSTRIDIUM-DIFFICILE-INFECTION; ENTAMOEBA-HISTOLYTICA/ENTAMOEBA-DISPAR; CLINICAL-PRACTICE GUIDELINES; ENZYME-IMMUNOASSAY; CRYPTOSPORIDIUM-PARVUM; ESCHERICHIA-COLI; GIARDIA-LAMBLIA; PARASITE PANEL; INTESTINAL PARASITES; EUROPEAN-SOCIETY;

Is the Medium Still the Message? Culture-Independent Diagnosis of Gastrointestinal Infections

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