A Comprehensive Genome-Wide Analysis of lncRNA Expression Profile during Hepatic Carcinoma Cell Proliferation Promoted by Phospholipase Cγ2

Xiaoguang Chen; Qiongxia Lv; Yumei Liu

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A Comprehensive Genome-Wide Analysis of lncRNA Expression Profile during Hepatic Carcinoma Cell Proliferation Promoted by Phospholipase Cγ2

机译：A Comprehensive Genome-Wide Analysis of lncRNA Expression Profile during Hepatic Carcinoma Cell Proliferation Promoted by Phospholipase Cγ2

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Phospholipase Cγ2 has the promotive effect on hepatic carcinoma development. Meanwhile, lncRNAs play a critical role in the pathology. Therefore, to make clear whether phospholipase Cyγ2 could enhance HCC cell proliferation by regulating lncRNA transcription, hepatic carcinoma cells RH35 were infected with Ad-phospholipase Cγ2 constructed previously, followed by lncRNA sequencing by high-throughput technology. Differently expressed lncRNAs (DElncRNAs) and their target genes were identified according to strict criteria. GO and KEGG, Reactome pathway analyses were performed for analyzing biological processes and the related pathways of DElncRNAs. lncRNA/mRNA coexpression pairs were screened according to expression profiling combined with bioinformatics analysis. The results showed that 231 DElncRNAs were identified in Ad-phospholipase Cγ2-overexpressing cells compared to control, containing 60 up- and 171 down-regulated ones. Target genes prediction analysis showed that 61 cis- and 30 transacting DElncRNAs were matched to 55 and 26 targets, respectively. Co-expression analysis found 33 lncRNA/mRNA coexpression pairs including 24 pairs in cis. GO analysis showed that these cw-mode lncRNA/mRNA pairs were involved in cytoskeleton organization, cell adhesion, and multiple signaling pathways related to apoptosis, proliferation and metastasis. Collectively, phospholipase Cγ2 caused the significant alterations in expression of many lncRNAs in liver cancer cells, providing valuable insight into precise mechanism of phospholipase Cγ2-promoting liver cancer cell growth.

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《Cytology and genetics》 |2023年第2期|186-196|共11页
作者
Xiaoguang Chen; Qiongxia Lv; Yumei Liu;
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Animal Science and Technology School, Henan University of Science and Technology, Luoyang, 471023 China;

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正文语种英语
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phospholipase Cγ2; hepatic carcinoma; lncRNA transcriptome; bioinformatic analysis;

A Comprehensive Genome-Wide Analysis of lncRNA Expression Profile during Hepatic Carcinoma Cell Proliferation Promoted by Phospholipase Cγ2

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