Immunogenicity and protective efficacy of a multi-epitope recombinant toxin antigen of Pasteurella multocida against virulent challenge in mice

Liang W.; Xiao H.; Chen J.-Y.Chang Y.-F.Cao S.-J.Wen Y.-P.Wu R.Du S.-Y.Yan Q.-G.Huang X.-B.Zhao Q.

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Immunogenicity and protective efficacy of a multi-epitope recombinant toxin antigen of Pasteurella multocida against virulent challenge in mice

机译：Immunogenicity and protective efficacy of a multi-epitope recombinant toxin antigen of Pasteurella multocida against virulent challenge in mice

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© 2023 Elsevier LtdPasteurella multocida (P. multocida) infection frequently results in porcine atrophic rhinitis and swine plague, leading to large economic losses for the swine industry worldwide. P. multocida toxin (PMT, 146 kDa) is a highly virulent key virulence factor that plays a vital role in causing lung and turbinate lesions. This study developed a multi-epitope recombinant antigen of PMT (rPMT) that showed excellent immunogenicity and protection in a mouse model. Using bioinformatics to analyse the dominant epitopes of PMT, we constructed and synthesized rPMT containing 10 B-cell epitopes, 8 peptides with multiple B-cell epitopes and 13 T-cell epitopes of PMT and a rpmt gene (1,974 bp) with multiple epitopes. The rPMT protein (97 kDa) was soluble and contained a GST tag protein. Immunization of mice with rPMT stimulated significantly elevated serum IgG titres and splenocyte proliferation, and serum IFN-γ and IL-12 were upregulated by 5-fold and 1.6-fold, respectively, but IL-4 was not. Furthermore, the rPMT immunization group exhibited alleviated lung tissue lesions and a significantly decreased degree of neutrophil infiltration compared with the control groups post-challenge. In the rPMT vaccination group, 57.1% (8/14) of the mice survived the challenge, similar to the bacterin HN06 group, while all the mice in the control groups succumbed to the challenge. Thus, rPMT could be a suitable candidate antigen for developing a subunit vaccine against toxigenic P. multocida infection.

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《Vaccine》 |2023年第14期|2387-2396|共10页
作者
Liang W.; Xiao H.; Chen J.-Y.Chang Y.-F.Cao S.-J.Wen Y.-P.Wu R.Du S.-Y.Yan Q.-G.Huang X.-B.Zhao Q.;
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Research Center of Swine Diseases College of Veterinary Medicine Sichuan Agricultural University;

Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine Cornell University;

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正文语种英语
中图分类医学免疫学;
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Epitope; Immunogenicity; Mice; Multi-epitope recombinant antigen of PMT (rPMT); Pasteurella multocida toxin (PMT);

Immunogenicity and protective efficacy of a multi-epitope recombinant toxin antigen of Pasteurella multocida against virulent challenge in mice

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