Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively

Chenqi Niu; Chong Zhang; Juewen Liu

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Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively

机译：Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively

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Estrogenic compounds such as estrone (El), 17/?-estradiol (E2), and 17α-ethynylestradiol (EE2) are serious environmental contaminants due to their potent biological activities. At least six selections were previously reported to obtain DNA aptamers for E2, highlighting its environmental importance. A careful analysis revealed that the previous aptamers either are too long or do not bind optimally. Herein, a series of new aptamers were obtained from the capture-SELEX method with dissociation constants down to 30 nM as determined by isothermal titration calorimetry (ITC). Two aptamers were converted to structure-switching fluorescent biosensors, which achieved a limit of detection down to 3.3 and 9.1 nM E2, respectively. One aptamer showed similar binding affinities to all the three estrogens, while the other aptamer is more selective for E2. Both aptamers required Mg~(2+) for binding. The proposed sensors were successfully applied in the determination of E2 in wastewater. Moreover, comparisons were made with previous aptamers based on primary sequence alignment and secondary structures. Among previously reported truncated aptamers, ITC showed binding only in one of them. The newly selected aptamers have the combined advantages of small size and high affinities.

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来源
《Environmental Science & Technology: ES&T》 |2022年第24期|17702-17711|共10页
作者
Chenqi Niu; Chong Zhang; Juewen Liu;
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作者单位

MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering Department of Chemical Engineering Tsinghua University, Beijing 100084, China;

Department of Chemistry, Waterloo Institute for Nanotechnology, Water Institute, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada;

MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering Tsinghua University, Beijing 100084, China;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类环境科学、安全科学;
关键词
estrogens; aptamers; water; biosensors; environmental monitoring;

Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively

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