Synthesis and Purification of N~3-Methylcytidine (m~3C) Modified RNA Oligonucleotides

Johnsi Mathivanan; Jinxi Du; Song MaoYa Ying ZhengJia Sheng

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Synthesis and Purification of N~3-Methylcytidine (m~3C) Modified RNA Oligonucleotides

机译：Synthesis and Purification of N~3-Methylcytidine (m~3C) Modified RNA Oligonucleotides

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This protocol describes a step-by-step chemical synthesis approach to prepare N~3-methylcytidine (m~3C) and its phosphoramidite. The method for synthesizing m~3C starts from commercially available cytidine, and proceeds via N~3-methylation in the presence of MeI, which generates the N~3-methylcytidine (m~3C) nucleoside, followed by the installation of several protecting groups at sites that include the 5'-hydroxyl group (4,4'-dimethoxytrityl protection), the 4-amino group (benzoyl protection), and the 2'-hydroxyl group ( tert-butyldimethylsilyl, TBDMS, protection). Standard phosphoramidite chemistry is applied to prepare the final m~3C phosphoramidite for solid-phase synthesis of a series of RNA oligonucleotides.

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来源
《Current Protocols in Nucleic Acid Chemistry》 |2021年第11期|e307-1-e307-13|共13页
作者
Johnsi Mathivanan; Jinxi Du; Song MaoYa Ying ZhengJia Sheng;
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作者单位

Department of Chemistry and the RNA Institute, University at Albany, State University of New York, Albany, New York;

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原文格式 PDF
正文语种英语
中图分类生物化学;
关键词
N3-methylcytidine; phosphoramidite; RNA modification; solid-phase synthesis;

Synthesis and Purification of N~3-Methylcytidine (m~3C) Modified RNA Oligonucleotides

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