Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange

Tang Xiaomei; Chen Shulin; Yu HuiwenZheng XiongjieZhang FeiDeng XiuxinXu Qiang

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Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange

机译：Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange

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Key message Here, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.

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来源
《Plant Cell Reports》 |2021年第12期|2453-2456|共4页
作者
Tang Xiaomei; Chen Shulin; Yu HuiwenZheng XiongjieZhang FeiDeng XiuxinXu Qiang;
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作者单位

Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan, Peoples R China;

Minnan Normal Univ, Key Lab Landscape Plants Fujian & Taiwan Characte, Zhangzhou 363000, Peoples R China;

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正文语种英语
中图分类植物细胞学;
关键词
PTG; Cas9; Sweet orange; Grafting; Heat stress; Efficient gene editing;

Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange

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