Insights into the discrepancy between Azadinium spp. and azaspiracid toxins near strategically important aquaculture operations in the west and southwest of Ireland

McGirr Stephen; Clarke Dave; Kilcoyne JaneSalas RafaelKoehler HenrySilke JoeTouzet Nicolas

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Insights into the discrepancy between Azadinium spp. and azaspiracid toxins near strategically important aquaculture operations in the west and southwest of Ireland

机译：Insights into the discrepancy between Azadinium spp. and azaspiracid toxins near strategically important aquaculture operations in the west and southwest of Ireland

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Apparent increases in harmful algal blooms worldwide have fostered attempts at mitigating their impacts on the aquaculture industry. The dinoflagellate species Azadinium spinosum has been described as the de novo azas-piracid (AZA) toxin producer of AZA-1 and-2 and been implicated in shellfish poisoning incidents (AZP) around Europe, regularly affecting shellfish mariculture operations. Several species of Azadinium have been confirmed in Irish coastal waters, and routine monitoring has shown disparities between Azadinum spp. cell count estimates in the water column and AZA concentrations in shellfish. A survey of bays on the southwest and west coasts of Ireland, carried out in August 2016, investigated the late summer distribution of Azadinium spp. and AZAs. Molecular analysis of water column samples showed very low levels of Az. spinosum. However, AZAs were found in 44% of samples, including in Az. spinosum negative samples. PCR-DGGE analysis was carried out using Amphidomataceae family specific primers on a selection of Az. spinosum negative, but AZA positive, samples. Subsequently sequenced DNA amplicons showed a high level of similarity with other Azadinium species suggesting that the species-specific molecular assays, in current use for monitoring Azadinium spp., are not capturing the likely greater diversity of the genus in Irish waters.

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《Estuarine coastal and shelf science》 |2021年第5期|107622.1-107622.9|共9页
作者
McGirr Stephen; Clarke Dave; Kilcoyne JaneSalas RafaelKoehler HenrySilke JoeTouzet Nicolas;
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作者单位

Inst Technol Sligo, Ctr Environm Res Innovat & Sustainabil, Sch Sci, Dept Environm Sci, Ash Lane, Sligo F91 YW50, Ireland;

Marine Inst, Shellfish Safety, Galway H91 R673, Ireland;

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正文语种英语
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West coast of Ireland; PCR-DGGE; Phytoplankton; AZAs; Azadinium; Real time PCR;

Insights into the discrepancy between Azadinium spp. and azaspiracid toxins near strategically important aquaculture operations in the west and southwest of Ireland

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