Effect of Short-Term and Long-Term UV-B Radiation on PSII Activity and Antioxidant Enzymes in Cucurbita pepo L. Leaves

Sezen Toksoy Koeseoglu; Ali Dogru

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Effect of Short-Term and Long-Term UV-B Radiation on PSII Activity and Antioxidant Enzymes in Cucurbita pepo L. Leaves

机译：Effect of Short-Term and Long-Term UV-B Radiation on PSII Activity and Antioxidant Enzymes in Cucurbita pepo L. Leaves

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This study aimed to investigate the antioxidant and photochemical responses of pumpkin (Cucurbita pepo L. cv. Kovas) plants under the short-term and long-term ultraviolet-B (UV-B) radiation. Pumpkin plants were exposed to 30 min (short-term) and 60 min (long-term) narrow-band UV-B (312 nm) radiation (UVBBE 2.88 kJ m~(-2)) from a 30 cm distance. Chlorophyll a fluorescence parameters were measured, and the JIP test was carried out. In addition, activities of some antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, glutathione reductase, and guaiacol peroxidase) were assayed and the content of free proline, total phenolic, anthocyanin, flavonoid, and photosynthetic pigments were determined. The long-term UV-B radiation reduced the efficiency of oxygen-evolving complex and electron flow from PSII reaction centers into the plastoquinone pool, induced accumulation of inactive reaction centers and non-photochemical quenching of the absorbed light energy in the leaves of pumpkin plants. Photosynthetic pigment content was kept at the control level, probably due to the remarkably increased anthocyanin content in the leaves of pumpkin plants. The decreased level of total soluble protein content may suggest oxidative degradation due to the inactivation of the ascorbate-glutathione cycle and H_2O_2 accumulation in leaf tissues. In addition, the accumulation of MDA in the leaves of pumpkin plants exposed to UV-B stress clearly showed severe membrane damage. As a result, it may be concluded that impairment in photosynthetic electron transport reactions in the leaves of pumpkin plants led to the unchanged APOD activity, accumulation of H_2O_2 and MDA, and consequently oxidative stress.

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《Biology bulletin》 |2022年第2期|S86-S101|共16页
作者
Sezen Toksoy Koeseoglu; Ali Dogru;
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Faculty of Science, Department of Biology, Sakarya University, Esentepe, Sakarya, 54187 Tuerkiye,Research-Development and Application Center (SARGEM), Sakarya University, Esentepe, Sakarya, 54187 Tuerkiye;

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antioxidant enzymes; chlorophyll a fluorescence; Cucurbita pepo; JIP test; photosynthesis; UV-B stress;

Effect of Short-Term and Long-Term UV-B Radiation on PSII Activity and Antioxidant Enzymes in Cucurbita pepo L. Leaves

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