Sublethal Xanthine Oxidase Stress Prefreezing of Bull Sperm Improves the Post-Thaw Functionality and Fertility Potential Parameters

Paniz Sedaghat; Reza Masoumi; Mohsen SharafiMaryam HezaveheiMaryam ShahverdiBehnam RostamiVahid Esmaeili

首页> 外文期刊>Biopreservation and biobanking >Sublethal Xanthine Oxidase Stress Prefreezing of Bull Sperm Improves the Post-Thaw Functionality and Fertility Potential Parameters

【24h】

Sublethal Xanthine Oxidase Stress Prefreezing of Bull Sperm Improves the Post-Thaw Functionality and Fertility Potential Parameters

机译：Sublethal Xanthine Oxidase Stress Prefreezing of Bull Sperm Improves the Post-Thaw Functionality and Fertility Potential Parameters

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Oxidative stress during cryopreservation causes mechanical, biochemical, and structural damage to the sperm, leading to lower viability and fertility potential. In recent years, a novel method based on the use of mild stress for preconditioning of sperm before cryopreservation has been applied to improve the quality of thawed sperm, although its molecular mechanism remains unknown. In this study, we investigated the protective effects of sublethal oxidative stress by xanthine oxidase (XO) on thawed bull sperm performance through modulations of mitochondrial uncoupling protein 2 (UCP2) expression. Semen samples were collected from six bulls, then mixed and divided into four aliquots: frozen control (XO-0) and frozen groups treated with different concentrations of XO, 0.01 μM (XO-0.01), 0.1 μM (XO-0.1), and 1 μM (XO-1). Thawed sperm were evaluated for motion parameters, viability, acrosome integrity, mitochondria activity, membrane integrity, and UCP2 expression. A significant increase of total motility and viability rate was observed in XO-0.1 compared with other frozen groups ( p < 0.05). The highest percentage of progressive motility was in XO-0.01 and XO-0.1 compared with other groups ( p < 0.05). Moreover, a significantly higher level of sperm mitochondrial membrane potential and membrane integrity was observed in XO-0.1 ( p < 0.05). We also found the lowest percentage of sperm mitochondria activity in XO-1 ( p < 0.05). In addition, the highest expression of UCP2 was observed in XO-1 ( p < 0.05). Our findings suggest that stress preconditioning of bull sperm before cryopreservation can improve thawed sperm functions, which might be mediated through an increase of UCP2 expression.

著录项

来源
《Biopreservation and biobanking》 |2023年第3期|288-293|共6页
作者
Paniz Sedaghat; Reza Masoumi; Mohsen SharafiMaryam HezaveheiMaryam ShahverdiBehnam RostamiVahid Esmaeili;
展开▼
作者单位

Department of Animal Science, University of Zanjan, Zanjan, Iran.;

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
bull semen; sublethal stress; xanthine oxidase; mitochondrial uncoupling protein 2; cryopreservation;

Sublethal Xanthine Oxidase Stress Prefreezing of Bull Sperm Improves the Post-Thaw Functionality and Fertility Potential Parameters

摘要

著录项

相关主题

期刊订阅