An efficient Agrobacterium-mediated transformation and regeneration protocol for aromatic black rice, Oryza sativa L. (cv. Chakhao Poireiton and Chakhao Amubi)

Ali Akib; Nongpiur Ramsong C. C.; Chrungoo Nikhil K. K.

首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >An efficient Agrobacterium-mediated transformation and regeneration protocol for aromatic black rice, Oryza sativa L. (cv. Chakhao Poireiton and Chakhao Amubi)

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An efficient Agrobacterium-mediated transformation and regeneration protocol for aromatic black rice, Oryza sativa L. (cv. Chakhao Poireiton and Chakhao Amubi)

机译：An efficient Agrobacterium-mediated transformation and regeneration protocol for aromatic black rice, Oryza sativa L. (cv. Chakhao Poireiton and Chakhao Amubi)

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Key messageThis article describes a time-efficient and high transformation efficiency protocol for Agrobacterium tumefaciens-mediated transformation of aromatic black rice cultivars, Chakhao Amubi and Chakhao Poireiton, for their genetic improvement. Crop-improvement efforts for Chakhao black rice (Oryza sativa L.) through genetic engineering have not made much progress due to the absence of a protocol for callus induction and regeneration. We developed a tissue culture protocol for the Agrobacterium-mediated transformation of Chakhao Amubi and Chakhao Poireiton cultivars of rice using mature seeds as the explants. The transformation protocol is simple and can be carried out in laboratories with basic equipment for tissue culture. The effect of temperature, growth regulators, gelling agents, and the duration of infection on different stages of the protocol is also described. Transgenic rice seedlings can be obtained in 69-80 days with > 30 transformation efficiency using the optimized method for each genotype.

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《Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants》 |2023年第3期|619-633|共15页
作者
Ali Akib; Nongpiur Ramsong C. C.; Chrungoo Nikhil K. K.;
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North Eastern Hill Univ;

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正文语种英语
中图分类植物细胞学;
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Black rice; Callus induction; Chakhao Amubi; Chakhao Poireiton; Rice transformation; Regeneration; PLANT-REGENERATION; CALLUS INDUCTION; SOMATIC EMBRYOGENESIS; GENOTYPIC VARIABILITY; ANTIOXIDANT CAPACITY; GELLING AGENTS; COLORED RICE; IDENTIFICATION; ANTHOCYANINS; TISSUE;

An efficient Agrobacterium-mediated transformation and regeneration protocol for aromatic black rice, Oryza sativa L. (cv. Chakhao Poireiton and Chakhao Amubi)

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