• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>iranian journal of microbiology >The molecular characterization of colistin-resistant isolates of Acinetobacter baumannii from patients at intensive care units
【24h】

The molecular characterization of colistin-resistant isolates of Acinetobacter baumannii from patients at intensive care units

机译：The molecular characterization of colistin-resistant isolates of Acinetobacter baumannii from patients at intensive care units

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

Background and Objectives: The objective of this study was to determine molecular characterization and genetic diversity of colistin-resistant A. baumannii clinical isolates in Intensive Care Unit hospitalized patients. Materials and Methods: A total of 127 A. baumannii clinical isolates were evaluated for antimicrobial susceptibility. PCR reaction and sequencing were performed for the detection of mutations in pmrAB and lpx ACD genes. Results: Based on antimicrobial susceptibility testing, 40.94 and 33.85 of the isolates were MDR and XDR respectively whereas 3.93 of them were found to be PDR. Results of agar dilution MIC and E-test indicated that 76 of the isolates were sensitive to colistin. All of the isolates were positive for bla(OXA-51) and 50 of them were positive for both bla(OXA-23)-like and bla(OXA-143)-like genes while only 25 of the isolates were positive for bla(OXA-72). None of them were positive for the bla(OXA-58)-like gene. There is no mutation in pmrA. The V162A substitution for pmrB gene was repeated in two isolates, and E394D and Y292H substitutions in lpxA were observed in two isolates; also, C-120 R and F165L substitutions in lpxC gene was repeated in two isolates. Analysis of phylogenetic tree based on alterations in lpxACD and pmrB genes indicated the appearance of new isolates compared to the reference strain ATCC17978 A. baumannii isolates. Conclusion: The present study indicated the prevalence of MDR and XDR A. baumannii isolates and the emergence of PDR isolates in the northwest portion of Iran. The appearance of colistin-resistant isolates with new mutations in pmrB, lpxACD genes indicates new resistance mechanisms.

著录项

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号