Fabrication of Biofunctionalized Protease-Based Chitosan/Collagen Composite Membranes and Efficient Biodegradation Using Recombinant Aspergillus Fumigatus

Richard A. Herman; Xuan Zhu; Ellen AyepaWen-Xin ZhangMichael AckahShuai YouJun Wang

首页> 外文期刊>Journal of polymers and the environment >Fabrication of Biofunctionalized Protease-Based Chitosan/Collagen Composite Membranes and Efficient Biodegradation Using Recombinant Aspergillus Fumigatus

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Fabrication of Biofunctionalized Protease-Based Chitosan/Collagen Composite Membranes and Efficient Biodegradation Using Recombinant Aspergillus Fumigatus

机译：Fabrication of Biofunctionalized Protease-Based Chitosan/Collagen Composite Membranes and Efficient Biodegradation Using Recombinant Aspergillus Fumigatus

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The present study aimed at developing chitosan/collagen protease-based composite membranes loaded with enzyme nanoparticles and accessing its efficient biodegradation using a recombinantly produced aspartic protease from Aspergillus fumigatus (AfAP) with functional biochemical properties. By using Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), and scanning electron microscopy (SEM) to analyze the resulting membranes, it was found that the ENPs and the chitosan-collagen composite membranes were highly compatible. Thermal properties (TG, DTG and DSC) were employed to evaluate the changes in the structural and physical properties which reached 384.94 ℃, and a residual mass of 4.21 ±0.13. The ENPs loaded membranes exhibited marginally improved antioxidant and microbial growth inhibitory activities reaching 57.29±2.77 and 10.33 ±0.65 mm respectively. Additionally, the respective protease employed for the degradation studies was successfully expressed in Pichia pastoris (GS115) host cells, with functional pH and temperature optima between pH 4.0-5.0 and 60 ℃ respectively, and specific activity of 8408.9±305.6 U/mg. It also exhibited enhanced specificity to its substrates upon collagen, chitosan, Bovine serum albumin (BSA), casein and casein sodium salt degradation with specific activities of 1477±99.1, 1177± 103, and 1051.6±60.7 U/mg respectively. Furthermore, the composite membranes showed excellent biodegradability by the 6th day in a natural environment treated with the aspartic protease from Aspergillus fumigatus (AfAP). Thus, the developed nanocomposite membranes from chitosan/ collagen exhibited great potential and can well mimic the functions of a new generation of biodegradable and sustainable membranes.

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《Journal of polymers and the environment》 |2023年第7期|3149-3166|共18页
作者
Richard A. Herman; Xuan Zhu; Ellen AyepaWen-Xin ZhangMichael AckahShuai YouJun Wang;
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Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, China;

College of Resources, Sichuan Agricultural University, Chengdu, Sichuan 611130, P.R. China;

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, P. R. ChinaSchool of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, P. R. China , Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science anJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212100, China , Key Laboratory of Silkworm and Mulberry Genetic Improvement, Sericultural Research;

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正文语种英语
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Chitosan; Collagen; Composite membranes; Enzyme nanoparticles; Biodegradation; Aspergillus fumigatus aspartic protease;

Fabrication of Biofunctionalized Protease-Based Chitosan/Collagen Composite Membranes and Efficient Biodegradation Using Recombinant Aspergillus Fumigatus

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