Regeneration peculiarities of in vitro berry cultures

E.V. Malaeva; O.I. Molkanova

摘要

Both Russian and foreign scientists carry out works on micropropagation of berry crops. For most berry crops, the method of micropropagation is developed quite effectively. However, in connection with the ever-changing range of berries, it is necessary to improve the technology of micropropagation at all stages of cultivation in vitro. In this regard, the aim of our research is to optimize the main stages of micropropagation of berry crops. The in vitro collection of berry crops of the Volgograd Regional Botanical Garden and the Main Botanical Garden named after N.V. Tsitsin of the RAS has more than 300 accessions and consists of representatives of the following families and genera: Grossulariaceae DC. (Grossularia Mill., Ribes L.) Caprifoliaceae Juss. (Viburnum L., Lonicera L.), Rosaceae Adans. (Rubus L., Cerasus Mill., Armeniaca Mill., Sorbus L., Prunus L.), Actinidiaceae Van-Tieghem (Actinidia Lindl.). The optimal period of isolation of berry crops explants is the phase of the beginning of active growth (April-May), while the yield of viable explants averaged 83. To obtain a sterile culture of fruit plants, in particular Actinidia, raspberries, honeysuckle, Lysofornin? 3000 was used at a concentration of 5, the exposure time was 3 min. When used, the yield of sterile explants was maximum and it was about 90. A comparative analysis of the study of the influence of different cytokinins on representatives of the genus Actinidia showed that when using 2 iP multiplication factor A. kolomikta and A. arguta significantly exceeded the value of this indicator in environments with 6-BAP. For A. polygama, the maximum value of the multiplication factor on media containing 6-BAP (0.5 mg L~(-1)) or zeatin (5.0 mg L~(-1)) was noted. For representatives of the genus Lonicera it is quite reasonable to use cytokinins in combination with auxins. The highest reproduction rate was observed at 6-BAP 1.0 mg L~(-1) + IBA 0.5 mg L~(-1), with a reproduction rate of 18±0.2. At the stage of rooting for Actinidia optimal concentration is IAA 1.0 mg L~(-1); for primocane raspberry - IAA 5.0 mg L~(-1); for honeysuckle - IBA 1.0 mg L~(-1). At the stage of adaptation of berry crops, the use of two-stage adaptation is very effective. The yield of adapted plants averaged 70-95.

Regeneration peculiarities of in vitro berry cultures

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