A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO2) nanocomposites for the detection of alpha-fetoprotein (AFP)

Kayani Farrukh Bashir; Rafique Saima; Akram RizwanHussain MozaffarBashir ShaziaNasir RubinaKhan Jan Sher

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A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO2) nanocomposites for the detection of alpha-fetoprotein (AFP)

机译：A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO2) nanocomposites for the detection of alpha-fetoprotein (AFP)

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Metal oxide-based sensors have the benefit of inexpensive, quick response, and high sensitivity in detecting specific biological species. In this article, a simple electrochemical immunosensor was fabricated using antibody-chitosan coated silver/cerium oxide (Ab-CS@Ag/CeO2) nanocomposites on a gold electrode for sensitive alpha-fetoprotein (AFP) diagnosis in human serum samples. Successfully synthesis of AFP antibody-CS@Ag/CeO2 conjugates was confirmed through Fourier transform infrared spectra of the prototype. The amine coupling bond chemistry was then used to immobilize the resultant conjugate on a gold electrode surface. It was observed that the interaction of the synthesized Ab-CS@Ag/CeO2 nanocomposites with AFP prevented an electron transfer and reduced the voltammetric Fe(CN)(6) (3-/4-) peak current, which was proportional to the amount of AFP. The linear ranges of AFP concentration were found from 10(-12)-10(-6) g.ml(-1). The limit of detection was calculated using the calibration curve and came out to be 0.57 pg.ml(-1). The designed label-free immunosensor successfully detected AFP in human serum samples. As a result, the resulting immunosensor is a promising sensor plate form for AFP detection and could be used in clinical bioanalysis.

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来源
《Nanotechnology》 |2023年第26期|共13页
作者
Kayani Farrukh Bashir; Rafique Saima; Akram RizwanHussain MozaffarBashir ShaziaNasir RubinaKhan Jan Sher;
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作者单位

Air Univ;

Pakistan Inst Engn & Appl Sci;

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原文格式 PDF
正文语种英语
中图分类特种结构材料;
关键词
immunosensor; alpha-fetoprotein (AFP); CS@Ag; CeO2 nanocomposites; cyclic voltammetry(CV); electrochemical impedance spectroscopy(EIS); NANOPARTICLES;

A simple, sensitive, label-free electrochemical immunosensor based on the chitosan-coated silver/cerium oxide (CS@Ag/CeO2) nanocomposites for the detection of alpha-fetoprotein (AFP)

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