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Antimicrobial activity of bioactive compounds of Haplopappus multifolius and Haplopappus taeda against human pathogenic microorganisms

机译:Antimicrobial activity of bioactive compounds of Haplopappus multifolius and Haplopappus taeda against human pathogenic microorganisms

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摘要

Background and Objectives: Haplopappus multifolius Phil. Ex Reiche and Haplopappus taeda Reiche are medicinal shrubs native to Chile and are popularly known as "Bailahuén". Regularly, this plant is used for liver, digestive and renal affections, as well as colds and the cleaning of infected wounds. The aim of the study was to identify the responsible compounds for the antimicrobial activity of H. multifolius and H. taeda. Materials and Methods: Infusions and ethanolic extracts of H. taeda and H. multifolius were analysed by thin-layer chromatography bioautography (TLC-B) to determine the compounds responsible for the antimicrobial activity against Gram-positive and Gram-negative bacterial strains and yeasts of Bailahuén. Finally, the minimum inhibitory concentration (MIC) of pure compounds isolated was determinate. Results: Extract of Bailahuén had activity only against Gram-positive bacterial strains and this activity was associated with aesculetin, 18-acetoxy-cis-cleroda-3,13E-dien-15-oic acid and aromadendrin-7-methyl ether compounds. Conclusion: H. multifolius and H. taeda have antibacterial capacity on different species of Gram-positive bacteria pathogenic for humans.

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