Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies

Mark D. Jankowski; David J. Fairbairn; Joshua A. BallerBenjamin M. WesterhoffHeiko L. Schoenfusse

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Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies

机译：Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies

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A major challenge in ecotoxicology is accurately and sufficiently measuring chemical exposures and biologicaleffects given the presence of complex and dynamic contaminant mixtures in surface waters. It is impractical to quantify allchemicals in such matrices over space and time, and even if it were practical, concomitant biological effects would not beelucidated. Our study examined the performance of the Daphnia magna transcriptome to detect distinct responses acrossthree water sources in Minnesota: laboratory (well) waters, wetland waters, and storm waters. Pyriproxyfen was included asa gene expression and male neonate production positive control to examine whether gene expression resulting fromexposure to this well‐studied juvenoid hormone analog can be detected in complex matrices. Laboratory‐reared (<24 h)D. magna were exposed to a water source and/or pyriproxyfen for 16 days to monitor phenotypic changes or 96 h toexamine gene expression responses using Illumina HiSeq 2500 (10 million reads per library, 50‐bp paired end 2 × 50).The results indicated that a unique gene expression profile was produced for each water source. At 119 ng/L pyriproxyfen(~25 effect concentration) for male neonate production, as expected, the Doublesex1 gene was up‐regulated. Indescending order, gene expression patterns were most discernable with respect to pyriproxyfen exposure status,season of stormwater sample collection, and wetland quality, as indicated by the index of biological integrity. However,the biological implications of the affected genes were not broadly clear given limited genome resources for invertebrates.Our study provides support for the utility of short‐term whole‐organism transcriptomic testing in D. magna to discernsample type, but highlights the need for further work on invertebrate genomics.

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《Environmental toxicology and chemistry /》 |2022年第9期|2107-2123|共17页
作者
Mark D. Jankowski; David J. Fairbairn; Joshua A. BallerBenjamin M. WesterhoffHeiko L. Schoenfusse;
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作者单位

Minnesota Pollution Control Agency, St. Paul, Minnesota, USA,Veterinary Population Medicine Department, University of Minnesota—Twin Cities, St. Paul, Minnesota, USA,US Environmental Protection Agency, Seattle, Washington, USA;

Minnesota Pollution Control Agency, St. Paul, Minnesota, USA;

Minnesota Supercomputing Institute, University of Minnesota—Twin Cities, Minneapolis, Minnesota, USAAquatic Toxicology Laboratory, St. Cloud State University, St. Cloud, Minnesota, USA;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类
关键词
Biomonitoring; Ecotoxicogenomics; Effects‐based monitoring; Invertebrate toxicology; Transcriptomics;

Using the Daphnia magna Transcriptome to Distinguish Water Source: Wetland and Stormwater Case Studies

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