Regulation of Colon Cancer Cells Biology by Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1(LncRNA MALAT1)via Targeting miR-184

Jun Bie; Junrong Zeng; Xinxiang Wu

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Regulation of Colon Cancer Cells Biology by Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1(LncRNA MALAT1)via Targeting miR-184

机译：Regulation of Colon Cancer Cells Biology by Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1(LncRNA MALAT1)via Targeting miR-184

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Colon cancer remains a serious threat to human,as IncRNAs and miRNAs are found as crucial components for colon cancer progression and metastasis.But the role of IncRNA MALAT1 in colon cancer remains elusive.Herein,we aimed at investigating its function in the colon cancer and exploring the interaction between MALAT1 and miR-184.We collected tumor tissue specimens from patients with colon cancer and determined the expression of MALAT1 and miR-184 using RT-qPCR.Functional experiment was established to detect the impact of MALAT1 siRNA or miR-184 inhibitor,followed by transwell assay and scratch experiment to assess colon cancer cell migration and invasion.Bioinformatics software and luciferase reporter system analyzed the targeting relationship between MALAT1 and miR-184.MALAT1 was up-regulated in colon cancer tissue,while miR-184 was poorly expressed and they were correlated with the clinicopathology of colon cancer(P<0.05).miR-184 was indicated as the target miRNA of MALAT1.Moreover,silencing of MALAT1 up-regulatedthe expression of miR-184 when activating Caspase3 activity,inhibiting Bcl-2 expression,increasing Bax expression.And MALAT1 siRNA inhibited cancer cell migration and invasion(P<0.05).However,transfection of miR-184 inhibitor abrogated these effectsinhibitor,when increasing MALAT1 expression.Collectively,we demonstrated that miR-184 is the target miRNA of Lnc-RNA MALAT1 and MALAT1 promotes colon cancer cell progression through inhibition of miR-184.

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《Journal of biomaterials and tissue engineering》 |2022年第11期|2153-2161|共9页
作者
Jun Bie; Junrong Zeng; Xinxiang Wu;
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作者单位

Department of Gastrointestinal Surgery,Xiantao First People's Hospital Affiliated to Yangtze University,Xiantao,433000,Hubei,China;

Department of Surgical Clinic,Xiantao First People's Hospital Affiliated to Yangtze University,Xiantao,433000,Hubei,China;

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正文语种英语
中图分类工程材料学;
关键词
LncRNA MALAT1; miR-184; Colon Cancer; Proliferation; Migration; Invasion;

Regulation of Colon Cancer Cells Biology by Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1(LncRNA MALAT1)via Targeting miR-184

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