Background and Objectives: Fungi communities are important soil components as decomposers and plant symbionts, and they play an important part in natural ecological and biogeochemical processes. In this study, isolation and identification of terrestrial and zoosporic fungi were detected.Materials and Methods: Sixty-seven fungal species from thirty-four genera were isolated from 45 soil samples obtained randomly from nurseries in Al-Qurayyat, Jouf reagon, Saudi Arabia using the soil dilution technique on glucose-Czapek's agar medium, cellulose-Czapek's agar, and Potato dextrose agar medium.Authentic fungus manuals were then used to identify and characterise the mycoflora.Results: A total of 46 fungal species belonging to 22 terrestrial fungal genera were recovered on glucose-Czapek's agar, 38 species belonging to 20 terrestrial fungal genera were recovered on cellulos-Czapek's agar and 27 fungal species belonging to 15 terrestrial fungal genera were recovered on PDA medium while 12 species belonging to 7 genera zoosporic fungal genera were discovered.Conclusion: The most common terrestrial fungal genera were Aspergillus, Penicillium, Fusarium, Trichoderma, Acremoni-um, and Cladosopium while in zoosporic fungus. Allomyces was the most prevalent, followed by Achlya and Pythium.
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