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首页> 外文期刊>Journal of insect biotechnology and sericology >19G1-30 kDa proteins in the membrane of the pupal fat body of Bombyx mori interact with a hemolymph chymotrypsin inhibitor
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19G1-30 kDa proteins in the membrane of the pupal fat body of Bombyx mori interact with a hemolymph chymotrypsin inhibitor

机译：Bombyx mori 蛹脂肪体膜中的 19G1-30 kDa 蛋白与血淋巴糜蛋白酶抑制剂相互作用

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© 2022, Japanese Society of Sericultural Sciences. All rights reserved.In the silkworm, Bombyx mori, there are 30 kDa proteins that are expressed in the fat body and secreted into the hemolymph. A 19G1-30 kDa protein in the membrane fraction of the late larval midgut is a probable receptor for the hemolymph chymotrypsin inhibitor CI-8. Take-up of CI-8 into the pupal perivisceral fat body suggests that the 19G1-30 kDa proteins function as a receptor in the fat body. We used biochemical assays to investi-gate whether the 19G1-30 kDa proteins were the receptor for CI-8 in the pupal fat body. The results of affinity purification, far western blotting, and western blotting suggested that the 19G1-30 kDa proteins are the interacting factors of the membrane fraction of the pupal fat body. Tissue surface analysis indicated that these proteins were the canonical tissue-surface proteins. Immunological analysis showed that, after the take-up of CI-8 into the fat body, dissociation of the 19G1-30 kDa protein and CI-8 occurred followed by aggregation of the 19G1-30 kDa proteins to form protein granules. The results showed that the 19G1-30 kDa protein is a receptor of CI-8.
机译：© 2022年，日本蚕桑学会。保留所有权利。在蚕 Bombyx mori 中，有 30 kDa 蛋白质在脂肪体中表达并分泌到血淋巴中。晚期幼虫中肠膜部分的 19G1-30 kDa 蛋白是血淋巴糜蛋白酶抑制剂 CI-8 的可能受体。CI-8 被摄取到蛹周围内脏脂肪体中表明 19G1-30 kDa 蛋白在脂肪体中起受体作用。我们使用生化分析来研究 19G1-30 kDa 蛋白是否是蛹脂肪体中 CI-8 的受体。亲和纯化、远西印迹和西印印结果表明，19G1-30 kDa蛋白是蛹脂肪体膜部分的相互作用因子。组织表面分析表明，这些蛋白是典型的组织表面蛋白。免疫学分析表明，CI-8被脂肪体吸收后，19G1-30 kDa蛋白和CI-8发生解离，随后19G1-30 kDa蛋白聚集形成蛋白颗粒。结果表明，19G1-30 kDa蛋白是CI-8的受体。

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