vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes

Fatemeh Habibinava; Mohammad Reza Zolfaghari; Mohsen ZargarSalehe Sabouri ShahrbabakMohammadMohammad Soleimani

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vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes

机译：vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes

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Background and Objectives: Multi-drug-resistant Enterobacter aerogenes is associated with various infectious diseases that cannot be easily treated by antibiotics. However, bacteriophages have potential therapeutic applications in the control of multi-drug-resistant bacteria. In this study, we aimed to isolate and characterize of a lytic bacteriophage that can lyse specifically the multi-drug-resistant (MDR) E. aerogenes. Materials and Methods: Lytic bacteriophage was isolated from Qaem hospital wastewater and characterized morphologically and genetically. Next-generation sequencing was used to complete genome analysis of the isolated bacteriophage. Results: Based on the transmission electron microscopy feature, the isolated bacteriophage (vB-Ea-5) belongs to the family Myoviridae . vB-Ea-5 had a latent period of 25 minutes, a burst size of 13 PFU/ml, and a burst time of 40 min. Genome sequencing revealed that vB-Ea-5 has a 135324 bp genome with 41.41 GC content. The vB-Ea-5 genome codes 212 ORFs 90 of which were categorized into several functional classes such as DNA replication and modification, transcriptional regulation, packaging, structural proteins, and a host lysis protein (Holin). No antibiotic resistance and toxin genes were detected in the genome. SDS-PAGE of vB-Ea-5 proteins exhibited three major and four minor bands with a molecular weight ranging from 18 to 50 kD. Conclusion: Our study suggests vB-Ea-5 as a potential candidate for phage therapy against MDR E. aerogenes infections.

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《Iranian Journal of Microbiology》 |2021年第2期|225-234|共10页
作者
Fatemeh Habibinava; Mohammad Reza Zolfaghari; Mohsen ZargarSalehe Sabouri ShahrbabakMohammadMohammad Soleimani;
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作者单位

Department of Microbiology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University;

Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences;

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical SciencesDepartment of Microbiology, School of Medicine, AJA University of Medical SciencesInfectious Diseases Research Center, AJA University of Medical Sciences;

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正文语种英语
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Drug resistance; Bacteriophages; Waste water; Enterobacter aerogenes; Myoviridae;

vB-Ea-5: a lytic bacteriophage against multi-drug-resistant Enterobacter aerogenes

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