Rapid and One-Step Screening of Taxane Compounds by a Two-Dimensional Carbon Microfiber Fractionation System Combinedwith Tandem Mass Spectrometry

Wang Zhao; Cui Meiyu; Ma BiaoYang LeiYu YingliCui HaiyanJin DongriShang HaiboLi Donghao

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Rapid and One-Step Screening of Taxane Compounds by a Two-Dimensional Carbon Microfiber Fractionation System Combinedwith Tandem Mass Spectrometry

机译：Rapid and One-Step Screening of Taxane Compounds by a Two-Dimensional Carbon Microfiber Fractionation System Combinedwith Tandem Mass Spectrometry

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Taxane compounds have attracted wide attention due to the basic chemical structure of taxol as an alternativeanticancer drug. The full-scan tandem mass spectrometry (MS/MS) fragmentation behaviors of seven taxane compounds werestudied. For taxanes of Sc-T and Sc-T-Xyl types, diagnostic product ions are originated from a cleavage in the ester bond of the C13position and the C-O bond of the C7position, and the subsequent fragmentation pattern is similar to those of M-type taxanes withthe loss of different numbers of acetic acid moieties (AcOH), benzoic acid moieties (BzOH), and H2O molecules. A rapid (7 min)and one-step screening method of two-dimensional microscale carbonfiber and active carbonfiber columns combined with tandemmass spectrometry (2D mu CFs-MS/MS) was developed for the screening of taxane compounds fromTaxus cuspidatasamples. BeforeMS/MS analysis, the 2D mu CFs system can group the sample extract without any pretreatment into three chromatographic-typefractions of strong, medium, and weak polarity to avoid matrix interference, such as lipids and pigments. The 2D mu CFs-MS/MS canalso conduct qualitative and quantitative analysis of taxane compounds, which is evaluated by limits of detection ranging from 3 to50 ng mL-1, limits of quantitation ranging from 10 to 150 ng mL-1, satisfactory recoveries from 75.2 to 112.2, and reproducibilitieswith relative standard deviations from 1.4 to 11.7.

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《Journal of Agricultural and Food Chemistry》 |2022年第15期|4774-4782|共9页
作者
Wang Zhao; Cui Meiyu; Ma BiaoYang LeiYu YingliCui HaiyanJin DongriShang HaiboLi Donghao;
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作者单位

Yanbian Univ;

Nankai Univ;

Yanbian Univ;

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正文语种英语
中图分类营养卫生、食品卫生;农业科学;
关键词
taxane; nbsp; 2D?CFs; rapid and one-step screening; Taxus cuspidata; PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE MICROEXTRACTION; 1ST TOTAL-SYNTHESIS; TAXOL; PLANT; PACLITAXEL; PHARMACEUTICALS; EXTRACTION; EFFICIENT; C-13;

Rapid and One-Step Screening of Taxane Compounds by a Two-Dimensional Carbon Microfiber Fractionation System Combinedwith Tandem Mass Spectrometry

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