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首页> 外文期刊>Environmental Science & Technology: ES&T >Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure
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Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

机译：Novel Use of Cell Profiling Technology to Visualize Mitochondrial Responses of Humpback Whale Fibroblasts to Chemical Exposure

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Cetaceansare at elevated risk of accumulating persistent and lipophilicenvironmental contaminants due to their longevity and high proportionof body fat. Despite this, there is a paucity of taxa-specific chemicaleffect data, in part due to the ethical and logistical constraintsin working with highly mobile aquatic species. Advances in cetaceancell culture have opened the door to the application of mainstream in vitro toxicological effect assessment approaches. Image-basedcell profiling is a high-throughput, microscopy-based system commonlyapplied in drug development. It permits the analysis of the xenobioticeffect on multiple cell organelles simultaneously, hereby flaggingits potential utility in the evaluation of chemical toxicodynamics.Here we exposed immortalized humpback whale skin fibroblasts (HuWa(TERT)) to six priority environmental contaminants known to accumulatein the Southern Ocean food web, in order to explore their subcellularorganelle responses. Results revealed chemical-dependent modulationof mitochondrial texture, with the lowest observed effect concentrationsfor chlorpyrifos, dieldrin, trifluralin, and p,p'-dichlorodiphenyldichloroethane of 0.3, 4.1, 9.3, and 19.8 nM, respectively.By contrast, no significant changes were observed upon exposure toendosulfan and lindane. This study contributes the first fixed mitochondrialimages of HuWa(TERT) and constitutes novel, taxa-specificchemical effect data in support of evidence-based conservation policyand management. Environmentalcontaminants impact on humpback whale bioenergeticsis unknown. This study provides mechanistic insight into the mitochondrialimpacts of known environmental contaminants, at environmentally relevantconcentrations, in humpback whales.

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