Engineering Densely Packed Adipose Tissue via Environmentally Controlled In-Bath 3D Bioprinting

Minjun Ahn; Won-Woo Cho; Byoung Soo KimDong-Woo Cho

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Engineering Densely Packed Adipose Tissue via Environmentally Controlled In-Bath 3D Bioprinting

机译：Engineering Densely Packed Adipose Tissue via Environmentally Controlled In-Bath 3D Bioprinting

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The adipose tissue is a crucial endocrine organ that coordinates with otherorgans, playing a key role in metabolic regulation. However, it remains challengingto recreate the morphology of native adipose tissue with fully packed adipogeniclipid droplets, mainly because of immature adipogenesis caused by the lowcell density of current adipose constructs. This study suggests environmentallycontrolled in-bath 3D bioprinting to create fully-mature densely packed adiposetissue (DPAT) in vitro. As a bath suspension, a hybrid bioink composed of 1alginate and 1.5 adipose-derived decellularized matrix is developed for selectiveand compact cell proliferation. In the hybrid bath suspension, the constructprinted at a high cell density (>10~7 cells ml~(?1)) proliferates within the predefinedarea without unwanted cell migration throughout the bath suspension, forminga densely packed cellular microenvironment. After adipogenesis for 4 weeks, theresults demonstrate that selectively proliferated preadipocytes can differentiateinto lipid-accumulating mature adipocytes. The resulting in vivo-like DPAT issuccessfully engineered in vitro. The DPAT construct shows the physiologicalchanges associated with obesity under the relevant conditions found in obesepatients. The recapitulation of obesity-induced inflamed adipose tissue throughco-culture with monocytes reveals the potency of the proposed strategy as apromising solution to overcome obesity-related complications.

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《Advanced functional materials》 |2022年第28期|2200203.1-2200203.13|共13页
作者
Minjun Ahn; Won-Woo Cho; Byoung Soo KimDong-Woo Cho;
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Department of Mechanical EngineeringPohang University of Science and Technology (POSTECH)Pohang, Kyungbuk 37673, Republic of Korea, POSTECH-Catholic Biomedical Engineering InstituteCollege of MedicineThe Catholic University of KoreaSeocho-gu, Seoul 06591,;

School of Biomedical Convergence EngineeringPusan National UniversityYangsan, Kyungnam 50612, Republic of Korea;

Department of Mechanical EngineeringPohang University of Science and Technology (POSTECH)Pohang, Kyungbuk 37673, Republic of Korea, Institute for Convergence Research and Education in Advanced TechnologyYonsei UniversitySeodaemun-gu, Seoul 03722, Republic;

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正文语种英语
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3D bioprinting; adipose tissue engineering; engineered adipose tissue; environmentally controlled; in-bath bioprinting; obesity-related inflammation; selective cell proliferation;

Engineering Densely Packed Adipose Tissue via Environmentally Controlled In-Bath 3D Bioprinting

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