Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase-2

Shun Zhang; Zhengpeng Wan; Georgios PavlouAmy X. ZhongLiling XuRoger D. Kamm

首页> 外文期刊>Advanced functional materials >Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase-2

【24h】

Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase-2

机译：Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase-2

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Self-organized microvascular networks (MVNs) have become key to the developmentof many microphysiological models. However, the self-organizingnature of this process combined with variations between types or batches ofendothelial cells (ECs) often lead to inconsistency or failure to form functionalMVNs. Because interstitial flow (IF) has been reported to play a beneficial rolein angiogenesis, vasculogenesis, and 3D capillary morphogenesis, the roleIF plays during neovessel formation in a customized single-channel microfluidicchip for which IF has been fully characterized is systematically investigated.Compared to static conditions, MVNs formed under IF have highervessel density and diameters and greater network perfusability. Through aseries of inhibitory experiments, this study demonstrates that IF treatmentimproves vasculogenesis by ECs through upregulation of matrix metalloproteinase-2 (MMP-2). This study then successfully implements a novel strategyinvolving the interplay between IF and MMP-2 inhibitor to regulate morphologicalparameters of the self-organized MVNs, with vascular permeabilityand perfusability well maintained. The revealed mechanism and proposedmethodology are further validated with a brain MVN model. The findings andmethods have the potential to be widely utilized to boost the developmentof various organotypic MVNs and can be incorporated into related bioengineeringapplications where perfusable vasculature is desired.

著录项

来源
《Advanced functional materials》 |2022年第43期|2206767.1-2206767.14|共14页
作者
Shun Zhang; Zhengpeng Wan; Georgios PavlouAmy X. ZhongLiling XuRoger D. Kamm;
展开▼
作者单位

Department of Biological EngineeringMassachusetts Institute of TechnologyCambridge, MA 02139, USA;

Ragon institute of MGHMIT and HarvardCambridge, MA 02139, USA;

Department of Biological EngineeringMassachusetts Institute of TechnologyCambridge, MA 02139, USA Department of Mechanical EngineeringMassachusetts Institute of TechnologyCambridge, MA 02139, USA;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类
关键词
blood–brain barrier; interstitial flow; matrix metalloproteinase-2; microphysiological system; microvascular networks; vasculogenesis;

Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase-2

摘要

著录项

相关主题

期刊订阅