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首页> 外文期刊>American Journal of Physiology >Media composition and O_2 levels determine effects of 17beta-estradiol and selective estrogen receptor modulators on mitochondrial bioenergetics and cellular reactive oxygen species
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Media composition and O_2 levels determine effects of 17beta-estradiol and selective estrogen receptor modulators on mitochondrial bioenergetics and cellular reactive oxygen species

机译：Media composition and O_2 levels determine effects of 17beta-estradiol and selective estrogen receptor modulators on mitochondrial bioenergetics and cellular reactive oxygen species

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Estradiol (E_2) and selective estrogen receptor modulators (SERMs) have broad-ranging cellular effects that include mitochondrial respiration and reactive oxygen species (ROS) metabolism. Many of these effects have been studied using cell culture models. Recent advances have revealed the extent to which cellular metabolism is affected by the culture environment. Cell culture media with metabolite composition similar to blood plasma e.g., Plasmax, Human Plasma-Like Medium (HPLM) alter cellular behaviors including responses to drugs. Similar effects have been observed with respect to O_2 levels in cell culture. Given these observations, we investigated whether the effects of E_2 and SERMs are also influenced by media composition and O_2 level during cell culture experiments. We analyzed mitochondrial network characteristics, cellular oxidative metabolism, and H_2O_2 production in C2C12 myoblasts growing in physiological (5) or standard cell culture (18)O_2 and in physiological (Plasmax) or standard cell culture Dulbecco's modified Eagle's medium (DMEM) media. Although E_2 significantly lowered H_2O_2 production from cells growing in 18 O_2/DMEM (standard cell culture), it had no effect on cells growing in Plasmax. Moreover, culture conditions significantly altered the effects of E_2 and SERMs on mitochondrial abundance and network characteristics. These results indicate that the effects of E_2 and SERMs on various aspects of cell physiology strongly depends on growth conditions, which in turn emphasizes the need to consider this carefully in cell culture experiments.

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