Mesenchymal stem cells suppressed skin and lung inflammation and fibrosis in topoisomerase I-induced systemic sclerosis associated with lung disease mouse model

Ganesan Nithya; Chang Yu-Di; Hung Shih-ChiehLan Joung-LiangLiao Jiunn-WangFu Shih TsungLee Chen-Chen

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Mesenchymal stem cells suppressed skin and lung inflammation and fibrosis in topoisomerase I-induced systemic sclerosis associated with lung disease mouse model

机译：Mesenchymal stem cells suppressed skin and lung inflammation and fibrosis in topoisomerase I-induced systemic sclerosis associated with lung disease mouse model

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Systemic sclerosis associated with lung interstitial lung disease (SSc-ILD) is the most common cause of death among patients with SSc. Mesenchymal stem cell (MSCs) transplantations had been treated by SSc patients that showed in the previous case report. The therapeutic mechanisms and effects of MSCs on SSc-ILD are still obscure. In this study, we investigated the therapeutic effects and mechanisms of treatment of BM-MSC derived from C57BL/6 on the topoisomerase I (TOPO I) induced SSc-ILD-like mice model. The mice were immunized with a mixture of recombinant human TOPO I in PBS solution (500 U/mL) and completed Freund's adjuvant CFA; 1:1 (volume/volume) twice per week for 9 weeks. On week 10, the mice were sacrificed to analyze the related pathological parameters. Lung and skin pathologies were analyzed using histochemical staining. CD4 T-helper (T-H) cell differentiation in lung and skin-draining lymph nodes was detected using flow cytometry. Our results revealed that allogeneic and syngeneic MSCs exhibited similar repressive effects on TOPO I-induced IgG1 and IgG2a in the SSc group. After intravascular (IV) treatment with syngeneic or allogeneic MSCs, the dermal thickness and fibrosis dramatically condensed and significantly reduced airway hyperresponsiveness. These findings showed that both allogeneic and syngeneic MSCs have therapeutic potential for SSc-ILD.

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来源
《Cell and Tissue Research》 |2023年第2期|323-337|共15页
作者
Ganesan Nithya; Chang Yu-Di; Hung Shih-ChiehLan Joung-LiangLiao Jiunn-WangFu Shih TsungLee Chen-Chen;
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作者单位

China Medical University Taiwan,China Med Univ;

Dept Internal Med,China Med Univ Hosp;

Grad Inst Vet Pathobiol,Natl Chung Hsing Univ;

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正文语种英语
中图分类细胞生物学;
关键词
Systemic sclerosis; Mesenchymal stem cells; Lung interstitial lung disease; Topoisomerase I and syngeneic or allogeneic; STROMAL CELLS; AUTOANTIBODY RESPONSE; PERSPECTIVES; SCLERODERMA; ENGRAFTMENT; CRITERIA;

Mesenchymal stem cells suppressed skin and lung inflammation and fibrosis in topoisomerase I-induced systemic sclerosis associated with lung disease mouse model

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