Depth-dependent variability of biological nitrogen fixation and diazotrophic communities in mangrove sediments

Zhiwen Luo; Qiuping Zhong; Xingguo HanRuiwen HuXingyu LiuWenjun XuYongjie WuWeiming HuangZhengyuan ZhouWei ZhuangQingyun YanZhili HeCheng Wang

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Depth-dependent variability of biological nitrogen fixation and diazotrophic communities in mangrove sediments

机译：Depth-dependent variability of biological nitrogen fixation and diazotrophic communities in mangrove sediments

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BACKGROUND:Nitrogen-fixing prokaryotes (diazotrophs) contribute substantially to nitrogen input in mangrove sediments, and their structure and nitrogen fixation rate (NFR) are significantly controlled by environmental conditions. Despite the well-known studies on diazotrophs in surficial sediments, the diversity, structure, and ecological functions of diazotrophic communities along environmental gradients of mangrove sediment across different depths are largely unknown. Here, we investigated how biological nitrogen fixation varied with the depth of mangrove sediments from the perspectives of both NFR and diazotrophic communities.RESULTS:Through acetylene reduction assay, nifH gene amplicon and metagenomic sequencing, we found that the NFR increased but the diversity of diazotrophic communities decreased with the depth of mangrove sediments. The structure of diazotrophic communities at different depths was largely driven by salinity and exhibited a clear divergence at the partitioning depth of 50 cm. Among diazotrophic genera correlated with NFR, Agrobacterium and Azotobacter were specifically enriched at 50-100 cm sediments, while Anaeromyxobacter, Rubrivivax, Methylocystis, Dickeya, and Methylomonas were more abundant at 0-50 cm. Consistent with the higher NFR, metagenomic analysis demonstrated the elevated abundance of nitrogen fixation genes (nifH/D/K) in deep sediments, where nitrification genes (amoA/B/C) and denitrification genes (nirK and norB) became less abundant. Three metagenome-assembled genomes (MAGs) of diazotrophs from deep mangrove sediments indicated their facultatively anaerobic and mixotrophic lifestyles as they contained genes for low-oxygen-dependent metabolism, hydrogenotrophic respiration, carbon fixation, and pyruvate fermentation.CONCLUSIONS:This study demonstrates the depth-dependent variability of biological nitrogen fixation in terms of NFR and diazotrophic communities, which to a certain extent relieves the degree of nitrogen limitation in deep mangrove sediments. Video Abstract.

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来源
《microbiome》 |2021年第1期|212-225|共14页
作者
Zhiwen Luo; Qiuping Zhong; Xingguo HanRuiwen HuXingyu LiuWenjun XuYongjie WuWeiming HuangZhengyuan ZhouWei ZhuangQingyun YanZhili HeCheng Wang;
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Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University;

Institute of Biogeochemistry and Pollutant Dynamics, Swiss Federal Institute of Technology;

South China Institute of Environmental Sciences, Ministry of Ecology and Environment;

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Depth-dependent variability; Diazotroph; Microbiome; Nitrogen cycling; Nitrogen fixation rate;

Depth-dependent variability of biological nitrogen fixation and diazotrophic communities in mangrove sediments

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