Improvement of in vitro growth and rooting of Magnolia grandi flora L. and Magnolia × soulangeana Soul.-Bod.

N. Dimitrova; L. Nacheva; V. IvanovaA. Medkov

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Improvement of in vitro growth and rooting of Magnolia grandi flora L. and Magnolia × soulangeana Soul.-Bod.

机译：Improvement of in vitro growth and rooting of Magnolia grandi flora L. and Magnolia × soulangeana Soul.-Bod.

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Magnolias are valuable ornamental plants for their showy flowers and foliage. They are also grown for their timber and potential applications as pharmaceuticals. Vegetative propagation could face some difficulties in root formation, transplanting and overwintering of the cuttings. Micropropagation could be a solution for these problems and could give the opportunity for mass production of good quality true-to-type plants. The success of in vitro cultivation of magnolias is highly genotype dependent and problems with low multiplication rate and weak rooting still remain unresolved in magnolia tissue culture. The objective of this study was to improve in vitro propagation of Magnolia. The influence of two cultural media based on both MS (Murashige and Skoog, 1962) or DKW (Driver and Kuniyuki, 1984), supplemented with cytokinin meta-topolin (mT) (0-8.5 μM) on multiplication of two Magnolia species - Magnolia grandiflora L. and Magnolia × soulangeana Soul.-Bod. was evaluated. The effect of the auxin indole-3-butyric acid (IBA) and the biostimulator Charkor on the rooting of microshoots of both species was tested. The best multiplication rate for both Magnolia species was achieved by enriching the nutrient medium with 7 μM mT. For Magnolia grandiflora L. DKW basal medium was more efficient, while for Magnolia × soulangeana Soul.-Bod. MS nutrient medium was better. Charkor biostimulator, added to the liquid MS nutrient medium, was highly efficient for the rooting of Magnolia grandiflora L. and Magnolia × soulangeana Soul.-Bod., as at a concentration of 1 mL L~(-1) 100 of rooting was achieved, regardless of the cytokinin (BAP or mT) used in the multiplication stage.

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来源
《Acta Horticulturae》 |2021年第1327期|349-360|共12页
作者
N. Dimitrova; L. Nacheva; V. IvanovaA. Medkov;
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作者单位

Fruit Growing Institute, 12 Ostromila Str., 4004 Plovdiv, Bulgaria;

Agricultural University, 12 Mendeleev Str., 4000, Plovdiv, Bulgaria;

NE ISTC "Agrobiotech", 50, Kharkivske shose, Kyiv, 02160, Ukraine;

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正文语种英语
中图分类园艺;
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micropropagation; nutrient medium; multiplication; adventitious root formation;

Improvement of in vitro growth and rooting of Magnolia grandi flora L. and Magnolia × soulangeana Soul.-Bod.

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