Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

Hao-Yu Liu; Antoine Giraud; Cedric SeignezDavid AhlFeilong GuoJohn SedinTomas WaldenJee-Hwan OhJan Peter van PijkerenLena HolmStefan RoosStefan BertilssonMia Phillipson

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Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

机译：Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

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BACKGROUND:Intestinal Peyer's patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation.RESULTS:The B cells of PPs were separated by size to circumvent activation-dependent cell identification biases due to dynamic expression of markers, which resulted in two phenotypically, transcriptionally, and spatially distinct subsets: small IgDsup+/sup/GL7sup-/sup/S1PR1sup+/sup/Bcl6, CCR6-expressing pre-germinal center (GC)-like B cells with innate-like functions located subepithelially, and large GL7sup+/sup/S1PR1sup-/sup/Ki67sup+/sup/Bcl6, CD69-expressing B cells with strong metabolic activity found in the GC. Peroral L. reuteri administration expanded both B cell subsets and enhanced the innate-like properties of pre-GC-like B cells while retaining them in the sub-epithelial compartment by increased sphingosine-1-phosphate/S1PR1 signaling. Furthermore, L. reuteri promoted GC-like B cell differentiation, which involved expansion of the GC area and autocrine TGFβ-1 activation. Consequently, PD-1-T follicular helper cell-dependent IgA induction and production was increased by L. reuteri, which shifted the intestinal microbiome and protected against dextran-sulfate-sodium induced colitis and dysbiosis.CONCLUSIONS:The Peyer's patches sense, enhance and transmit probiotic signals by increasing the numbers and effector functions of distinct B cell subsets, resulting in increased IgA production, altered intestinal microbiota, and protection against inflammation. Video abstract.

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来源
《microbiome》 |2021年第1期|198-215|共18页
作者
Hao-Yu Liu; Antoine Giraud; Cedric SeignezDavid AhlFeilong GuoJohn SedinTomas WaldenJee-Hwan OhJan Peter van PijkerenLena HolmStefan RoosStefan BertilssonMia Phillipson;
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作者单位

Laboratory of Animal Physiology and Molecular Nutrition, College of Animal Science and Technology, Yangzhou University;

Department of Medical Cell Biology, Uppsala University;

Department of Food Science, University of Wisconsin-MadisonDepartment of Molecular Sciences, Swedish University of Agricultural SciencesDepartment of Aquatic Sciences and Assessment, Swedish University of Agricultural SciencesScience for Life Laboratory;

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正文语种英语
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Gut microbiome; Inflammatory bowel disease; Innate-like B lymphocytes; PD-1 dependent; Probiotics; R2LC;

Distinct B cell subsets in Peyer’s patches convey probiotic effects by Limosilactobacillus reuteri

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