Syntheses of Specifically ~(15)N-Labeled Adenosine and Guanosine

Roger A. Jones; Barbara L. Gaffney

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Syntheses of Specifically ~(15)N-Labeled Adenosine and Guanosine

机译：Syntheses of Specifically ~(15)N-Labeled Adenosine and Guanosine

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This article describes the specific incorporation of ~(15)N into the N7 and amino positions of adenosine (Basic Protocol 1), and conversion of the adenosine to guanosine labeled at the N1, N7, and amino positions (Basic Protocol 2). Two variations of the procedures are also presented that include either ~(12)C or ~(13)C at the C8 position of adenosine, and ~(13)C at either the C8 or C2 position of guanosine. These ~(13)C tags permit the incorporation of two ~(15)N-labeled nucleosides into an RNA strand while ensuring that their nuclear magnetic resonance (NMR) signals can be distinguished from each other by the presence or absence of C-N coupling. While the major application of these specifically ~(15)N-labeled nucleosides is NMR, the additional mass makes them useful in mass spectrometry (MS) as well. The procedures can also be adapted to synthesize the labeled deoxynucleosides. The Support Protocol describes the synthesis of 7-methylguanosine.

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《Current Protocols in Nucleic Acid Chemistry》 |2022年第12期|e612-1-e612-15|共15页
作者
Roger A. Jones; Barbara L. Gaffney;
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作者单位

Chemistry and Chemical Biology, Rutgers University New Brunswick, New Brunswick, New Jersey;

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原文格式 PDF
正文语种英语
中图分类生物化学;
关键词
carbon-13 labeled nucleosides; labeled nucleosides; nitrogen-15 labeled nucleosides; ~(15)N-labeled adenosine; ~(15)N-labeled guanosine;

Syntheses of Specifically ~(15)N-Labeled Adenosine and Guanosine

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