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Folate Receptor-Targeted NIR-Ⅱ Dual-Model Nanoprobes for Multiscale Visualization of Macrophages in Rheumatoid Arthritis

机译:Folate Receptor-Targeted NIR-Ⅱ Dual-Model Nanoprobes for Multiscale Visualization of Macrophages in Rheumatoid Arthritis

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摘要

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized byjoint inflammation. Macrophages accelerate the pathological process of RAthrough the continuous cell accumulation and secretion of cytokines. Therefore,multiscale visualization of macrophages is of great significance for theearly diagnosis of RA. In this work, folate receptor-targeted Cptnc-4F nanoprobes,named FA-CF-NPs, are developed for dual-model molecular imaging ofmacrophages in RA mouse models. FA-CF-NPs exhibit complementary imagingadvantages with high fluorescence sensitivity and resolution in the secondnear-infrared window (NIR-II) and promising photoacoustic (PA) imagingcontrast with deep tissue penetration. The high affinity of the folate ligand onthe surface of FA-CF-NPs to macrophages with overexpressed folate receptorsensures the specific targeting function of the nanoprobes for imaging in vivo.FA-CF-NPs are employed to achieve NIR-II fluorescence imaging to accuratelylocalize macrophages in the joint, obtaining a high signal-to-background ratio(SBR) of 15.5. Meanwhile, PA imaging is also performed to observe macrophagedistribution in the longitudinal section of the whole joint. Moreover,macrophage infiltration is detected in the joint of RA mice with the absenceof RA pathologies such as synovitis and cartilage loss. The results provide anopportunity for in vivo macrophage tracking and early diagnosis of RA.

著录项

  • 来源
    《Advanced functional materials》 |2023年第29期|2300342.1-2300342.11|共11页
  • 作者单位

    Department of Materials Science and EngineeringShenzhen Key Laboratory of Printed Organic ElectronicsSouthern University of Science and TechnologyShenzhen 518055, P. R. China;

    Paul C. Lauterbur Research Center for Biomedical ImagingInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055, P. R. China;

    Department of Materials Science and EngineeringShenzhen Key Laboratory of Printed Organic ElectronicsSouthern University of Science and TechnologyShenzhen 518055, P. R. China;

    Research Laboratory for Biomedical Optics and Molecular ImagingGuangdong ProvincDepartment of UltrasoundThe First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhou 510405, P. R. ChinaGuangzhou University of Chinese MedicineGuangzhou 510006, P. R. China;

    Paul C. Lauterbur Research Center for Biomedical ImagingInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055Paul C. Lauterbur Research Center for Biomedical ImagingInstitute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055, P. R. China,Shenzhen College of Advanced TechnologyUniversity of Chi;

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  • 原文格式 PDF
  • 正文语种 英语
  • 中图分类
  • 关键词

    fluorescence imaging; macrophages; photoacoustic imaging; rheumatoid arthritis; second near-infrared windows;

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