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首页> 外文期刊>Environmental Science & Technology: ES&T >Molecular and Dual-lsotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge
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Molecular and Dual-lsotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge

机译：Molecular and Dual-lsotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge

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摘要

Nitrate (NO3-) leaching is a serious health and ecological concern in global agroecosystems, particularly those under the application of agricultural-managed aquifer recharge (Ag-MAR); however, there is an absence of information on microbial controls affecting NO3-leaching outcomes. We combine natural dual isotopes of NO3-(1SN/14N and 18O/16O) with metagenomics, quantitative polymerase chain reaction (PCR), and a threshold indicator taxa analysis (TITAN) to investigate the activities, taxon profiles, and environmental controls of soil microbiome associated with N03~ leaching at different depths from Californian vineyards under Ag-MAR application. The isotopic signatures demonstrated a significant priming effect (P < 0.01) of Ag-MAR on denitrification activities in the topsoil (0 10 cm), with a 12 25-fold increase of 1SN N03~ and I80 N03~ after the first 24 h of flooding, followed by a sharp decrease in the enrichment of both isotopes with ~80 decline in denitrification activities thereafter. In contrast, deeper soils (60 100 cm) showed minimal or no denitrification activities over the course of Ag-MAR application, thus resulting in 10 20-fold of residual N03~ being leached. Metagenomic profiling and laboratory microcosm demonstrated that both nitrifying and denitrifying groups, responsible for controlling N03~ leaching, decreased in abundance and potential activity rates with soil depth. TITAN suggested that Nitrosocosmicus and Bradyrhizobiwn, as the major nitrifier and denitrifier, had the highest and lowest tipping points with regard to the N03~ changes (P < 0.05), respectively. Overall, our study provides new insight into specific depth limitations of microbial controls on soil N03~ leaching in agroecosystems.

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