A simple apparatus is described for bringing a protein or a nucleic acid molecule abruptly from an H2O medium into a D2O medium and at the same time to introduce the resultant D2O solution rapidly in a sealed cell for an infrared absorption measurement. It has a small Sephadex column and three magnet valves, and biopolymer molecules are forced to pass the column and pushed into the infrared cell with D2O by means of an external pressure of nitrogen gas. By the use of this apparatus an infrared absorption measurement can be started at 10 sec from the ``zerohyphen;time,'' when the biopolymer molecules are brought into contact with the D2O molecules.
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