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>Demonstration of 1α,25‐dihydroxyvitamin D3receptor‐like molecule in ST 13 and 3T3 L1 preadipocytes and its inhibitory effects on preadipocyte differentiation
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Demonstration of 1α,25‐dihydroxyvitamin D3receptor‐like molecule in ST 13 and 3T3 L1 preadipocytes and its inhibitory effects on preadipocyte differentiation
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机译：Demonstration of 1α,25‐dihydroxyvitamin D3receptor‐like molecule in ST 13 and 3T3 L1 preadipocytes and its inhibitory effects on preadipocyte differentiation
AbstractThe active metabolite of vitamin D3, 1α,25‐dihydroxyvitamin D3(1,25(OH)2D3), inhibited morphologic and enzymatic expression during differentiation of preadipocyte to adipocyte. In the presence of ∼6.4–20 × 10−10M 1,25(OH)2D3, the triacylglycerol accumulation was only 50 of that of fully differentiated control cells. High‐affinity binding sites for 1,25(OH)2D3binding component sediments at 3.3 S in 4–24 (w/v) sucrose gradients prepared in hypertonic buffer. Binding assay revealed that Nmaxwas 70 fmol/mg protein and 90 fmol/mg protein, and Kdvalue was 170 pM and 37 pM in cell lines ST 13 and 3T3 L1, respectively. We also found that differentiated adipocytes did not contain specific receptors for 1,25(OH)2D3. 1,25(OH)2D3, 1(OH)D3, 24,25(OH)2D3, and 24(OH)D3all suppressed differentiation of preadipocytes to adipocytes, and the dose required closely reflected the affinities of the various metabolites and the synthetic derivative for 1,25(OH)2D3receptor. It is suggested that the action of vitamin D3on preadipocyte differentiation may result from a receptor
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