Abstract:In this study an attempt was made to find an optimum method of chemical treatment to prevent the calcification of bioprosthetic heart valves. Bovine pericardium was washed in a 5 sodium chloride solution followed by trypsin (Tr) treatment and was kept in 0. 1 glutaraldehyde (GA) with a gradual increase in concentration up to 0. 25 GA and finally posttreated with a 4 chitosan (Ch) solution. Fresh, 0. 2 GA, 0. 625 GA, and sodium chloride–Tr–GA treated pericardial samples were taken for comparative study. Tensile testing showed comparable strength and elongation at the breaking point for all groups. The thermal shrinkage studies indicated merit of the proposed treatment (5 sodium chloride–trypsinglutaraldehyde treated pericardia with chitosan and without chitosan posttreatment). Collagenase assay showed that all differently treated (GA) materials were equally resistant to collagenase. All samples were implanted subcutaneously in rats for 2, 4, 8, or 12 weeks for calcification study. Morphological and mineral analyses showed complete prevention of calcification in sodium chloride–trypsin–GA–chitosan treated pericardium (Ca was 1. 1 ± 0. 27 mg/g, von Kossa 0) at the 12th week of
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