A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin.

Cobos Marin L; Montes Vargas J; Rivera Gutierrez SLicea Navarro AGonzalez y Merchand JAEstrada Garcia I

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A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin.

机译：A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin.

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摘要

Bovine tuberculosis is a zoonotic disease that not only causes huge economic losses but also poses an important risk for human infection. The definitive identification of a clinical isolate relies on time-consuming, highly specialized and laborious biochemical tests. We have developed a method for the rapid and reliable identification of Mycobacterium bovis and for its simultaneous differentiation from other members of the M. tuberculosis complex. Furthermore, the technique also allowed us to distinguish M. tuberculosis complex members from other Mycobacterial species. The method comprises both a single PCR and a multiplex-PCR and can be confidently applied to samples of both veterinary and human origin.

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来源
《Epidemiology and Infection》 |2003年第3期|485-490|共6页
作者
Cobos Marin L; Montes Vargas J; Rivera Gutierrez SLicea Navarro AGonzalez y Merchand JAEstrada Garcia I;
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作者单位

Departamento de Inmunologia, Escuela Nacional de Ciencias Biologicas, IPN;

Prolongacion Carpio y Plan de Ayala s/n, Col. Sto. Tomas, Mexico DF, 11340, Mexico.;

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原文格式 PDF
正文语种英语
中图分类内科学;
关键词
Bacterial Typing Techniques; Mycobacterium bovis; Polymerase Chain Reaction; 细菌分型技术; 分支杆菌; 牛; 聚合酶链反应; 结核;

A novel multiplex-PCR for the rapid identification of Mycobacterium bovis in clinical isolates of both veterinary and human origin.

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