The genes coding for toluene monooxygenase (TMO), an enzyme also capable of degrading trichloroethylene (TCE), fromPseudomonas mendocinaKR1 were cloned into both narrow#x2010; and broad#x2010;host#x2010;range vectors and introduced intoEscherichia coliandP. putida.TMO activity expressed by a number of strain/plasmid combinations was assayed by determining decreases in the fluorescence of indble. Degradation of TCE was investigated in sealed serum vials for a number of these combinations. Finally a kinetic experiment, involving determination of the rate of TCE degradation at various TCE concentrations, was performed, and approximate kinetic constants for TCE degradation by these two species were determined.
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