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Effect of Weak Acid Hypochlorous Solution on Selected Viruses and Bacteria of Laboratory Rodents

机译：弱酸次氯溶液对实验室啮齿动物特定病毒和细菌的影响

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摘要

Weak acid hypochlorous solution (WAHS) is known to have efficacy for inactivating pathogens and to be relatively safe with respect to the live body. Based on these advantages, many animal facilities have recently been introducing WAHS for daily cleaning of animal houses. In this study, we determined the effect of WAHS in inactivating specific pathogens of laboratory rodents and pathogens of opportunistic infection. WAHS with an actual chloride concentration of 60 ppm and a pH value of 6.0 was generated using purpose-built equipment. One volume of mouse hepatitis virus (MHV), Sendai virus, lymphocytic choriomeningitis virus, Bordetella bronchiseptica, Pasteurella pneumotropica, Cotynebacterium kutscheri, Staphylococcus aureus, and Pseudomonas aeruginosa was mixed with 9 or 99 volumes of WAHS (x 10 and x100 reaction) for various periods (0.5, 1, and 5 min) at 25 degrees C. After incubation, the remaining infectious viruses and live bacteria were determined by plaque assay or culture. In the x100 reaction mixture, infectious viruses and live bacteria could not be detected for any of the pathogens examined even with the 0.5-min incubation. However, the effects for MHV, B. bronchiseptica, and P. aeruginosa were variable in the x10 reaction mixture with the 0.5- and 1-min incubations. Sufficient effects were obtained by elongation of the reaction time to 5 min. In the case of MHV, reducing organic substances in the virus stock resulted in the WAHS being completely effective. WAHS is recommended for daily cleaning in animal facilities but should be used properly in order to obtain a sufficient effect, which includes such things as using a large enough volume to reduce effects of organic substances.
机译：已知弱酸次氯溶液 （WAHS） 具有灭活病原体的功效，并且对活体相对安全。基于这些优势，许多动物设施最近都引入了WAHS来日常清洁动物舍。在这项研究中，我们确定了 WAHS 在灭活实验室啮齿动物的特定病原体和机会性感染病原体方面的作用。使用专用设备生成实际氯浓度为 60 ppm、pH 值为 6.0 的 WAHS。将一体积的小鼠肝炎病毒 （MHV）、仙台病毒、淋巴细胞性脉络丛脑膜炎病毒、支气管败血博德特氏菌、嗜肺巴斯德氏菌、库氏子杆菌、金黄色葡萄球菌和铜绿假单胞菌与 9 或 99 体积的 WAHS（x 10 和 x100 反应）混合，在 25 °C 下进行不同时间段（0.5、1 和 5 分钟）。孵育后，通过噬菌斑测定或培养测定剩余的感染性病毒和活细菌。在 x100 反应混合物中，即使孵育 0.5 分钟，也无法检测到任何病原体的传染性病毒和活细菌。然而，在 0.5 分钟和 1 分钟孵育的 x10 反应混合物中，对 MHV、支气管败血杆菌和铜绿假单胞菌的影响是可变的。通过将反应时间延长至5分钟获得足够的效果。在MHV的情况下，减少病毒库存中的有机物质导致WAHS完全有效。建议在动物设施中进行日常清洁，但应正确使用以获得足够的效果，其中包括使用足够大的体积以减少有机物质的影响。

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