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首页> 外文期刊>cell and tissue research >Improved visualization of the immunoreactive hypothalamo-neurohypophysial system by use of immuno-gold techniques
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Improved visualization of the immunoreactive hypothalamo-neurohypophysial system by use of immuno-gold techniques

机译：Improved visualization of the immunoreactive hypothalamo-neurohypophysial system by use of immuno-gold techniques

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摘要

Ultrastructural post-embedding immuno-gold techniques were applied to the supraoptic nucleus and the neurohypophysis of mice and rats. The primary antibodies were three different monoclonal antineurophysins, used in protein A-gold and immunoglobulin-gold procedures. Conventional plastic embedding as well as hydrophilic media (L.R. White) were used; non-osmicated and osmicated tissues were immunolabeled; sodium metaperiodate oxidation was used, but was not essential for immunolabeling.

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  • 来源
    《cell and tissue research》 |1986年第1期|193-204|共页
  • 作者

    M.Castel; J.F.Morris; M.H.WhitnallN.Sivan;

  • 作者单位

    Electron Microscopy Unit, Institute of Life Sciences, Hebrew University of Jerusalem;

    Oxford University;

    Laboratory of Neurochemistry and Neuroimmunology, NICHD, NIH;

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