Interactions of the Hindgut Mucosa-Associated Microbiome with Its Host Regulate Shedding of Escherichia coli O157:H7 by Cattle

Wang Ou; McAllister Tim A.; Plastow GrahamStanford KimSelinger BrentGuan Le Luo

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Interactions of the Hindgut Mucosa-Associated Microbiome with Its Host Regulate Shedding of Escherichia coli O157:H7 by Cattle

机译：Interactions of the Hindgut Mucosa-Associated Microbiome with Its Host Regulate Shedding of Escherichia coli O157:H7 by Cattle

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Cattle are the primary carrier of Escherichia coli O157: H7, a foodborne human pathogen, and those shedding 10(4) CFU/gram of feces of E. coli O157: H7 are defined as supershedders (SS). This study investigated the rectoanal junction (RAJ) mucosa-associated microbiota and its relationship with host gene expression in SS and in cattle from which E. coli O157: H7 was not detected (nonshedders NS), aiming to elucidate the mechanisms involved in supershedding. In total, 14 phyla, 66 families, and 101 genera of RAJ mucosa-associated bacteria were identified and Firmicutes (61.5 +/- 7.5), Bacteroidetes (27.9 +/- 6.4), and Proteobacteria (5.5 +/- 2.1) were the predominant phyla. Differential abundance analysis of operational taxonomic units (OTUs) identified 2 OTUs unique to SS which were members of Bacteroides and Clostridium and 7 OTUs unique to NS which were members of Coprococcus, Prevotella, Clostridium, and Paludibacter. Differential abundance analysis of predicted microbial functions (using PICRUSt phylogenetic investigation of communities by reconstruction of unobserved states) revealed that 3 pathways had higher abundance (log2 fold change, 0.10 to 0.23) whereas 12 pathways had lower abundance (log(2) fold change, -0.36 to -0.20) in SS. In addition, we identified significant correlations between expression of 19 differentially expressed genes and the relative abundance of predicted microbial functions, including nucleic acid polymerization and carbohydrate and amino acid metabolism. Our findings suggest that differences in RAJ microbiota at both the compositional and functional levels may be associated with E. coli O157: H7 supershedding and that certain microbial groups and microbial functions may influence RAJ physiology of SS by affecting host gene expression.

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《Applied and Environmental Microbiology》 |2018年第1期|共15页
作者
Wang Ou; McAllister Tim A.; Plastow GrahamStanford KimSelinger BrentGuan Le Luo;
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作者单位

Alberta Agr & Forestry, Lethbridge, AB, Canada;

Univ Lethbridge, Dept Biol Sci, Lethbridge, AB, Canada;

Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, CanadaAgr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB, Canada;

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原文格式 PDF
正文语种英语
中图分类应用微生物学;环境科学基础理论;
关键词
cattle; E. coli O157: H7; rectoanal junction; microbiome; gene expression; E. coli O157;

Interactions of the Hindgut Mucosa-Associated Microbiome with Its Host Regulate Shedding of Escherichia coli O157:H7 by Cattle

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