AbstractA novel scheme for the separation and live recovery of one cell type from a mixture of cells using a cell affinity chromatography (CAC) system is demonstrated. An antimurine IgG was chemically immobilized to a cellophane support via a carbonyldiimidazole (CDI) link. Murine splenocytes flowed over the support, and B‐cells were allowed to attach at a shear rate of 15 s−1. Once loading was terminated, the support was washed at a shear rate of 315 s−1to remove nonspecifically bound cells. Elution of the B‐cells was initiated by the transmembrane diffusion of hydrochloric acid (pH 1), supplied to the side of the membrane opposite the cells. At the same time, a shear flow of normal saline was established on the cell side of the membrane, and cells, freed by acid, were retrieved. Results showed that, on average, 250 cells/mm2attached to antibody immobilized on cellophane surfaces, at a shear rate of 15 s−1, and that attached cells were successfully displaced by acid supplied to the side of the membrane opposite that holding the cells. On average, at least 60 of the B‐cells removed by this elution appeared viable, based on a Trypan Blue dye excl
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