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首页> 外文期刊>journal of cellular biochemistry >Cell transformation by c‐Ha‐rasVal12oncogene is accompanied by a decrease in histone H1° and an increase in nucleosomal repeat length
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Cell transformation by c‐Ha‐rasVal12oncogene is accompanied by a decrease in histone H1° and an increase in nucleosomal repeat length

机译：Cell transformation by c‐Ha‐rasVal12oncogene is accompanied by a decrease in histone H1° and an increase in nucleosomal repeat length

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AbstractThe activated c‐Ha‐rasVal12oncogene is often involved in the genesis of human malignancies. We show here that in c‐Ha‐rasVal12oncogene‐transformed mouse NIH 3T3 fibroblasts the copy number and expression level of the mutantrasoncogene correlates with the degree of chromatin decondensation, as assessed by micrococcal nuclease (MNase) and DNase I digestion. MNase and DNase I analyses further revealed that the nucleosomal repeat lengths were different in the normal andrasoncogene‐transformed cells, 162.3 bp and 178.1 bp, respectively. These chromatin changes were accompanied by alterations in the content of histone H1°. Furthermore, using DNase I as a probe, we discovered that serum stimulation of normal and transformed cells, synchronized by serum starvation, induces rapid reversible changes in the structure of bulk chromatin that may be linked to transcriptional activation. Our data thus indicate that cell transformation byrasis associated with specific changes in chromatin structure that make it more vulnerable, and prone to additional mutations characteristic of cancer develop

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