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>Sequential conversion of cortexolone to prednisolone by immobilized mycelia ofCurvularia lunataand immobilized cells ofArthrobacter simplex
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Sequential conversion of cortexolone to prednisolone by immobilized mycelia ofCurvularia lunataand immobilized cells ofArthrobacter simplex
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机译：Sequential conversion of cortexolone to prednisolone by immobilized mycelia ofCurvularia lunataand immobilized cells ofArthrobacter simplex
Two-step bioconversion of cortexolone (Reichstein's Compound S) to itsΔ1-dehaydro-11β-hydroxy derivative, prednisolone, was successfully performed by the combined use of immobilizedCurvularia lunatamycelia and immobilizedArthrobacter simplexcells. Immobilized living mycelia ofC. lunatahaving a high 11β-hydroxylation activity were prepared byin situgermination of spores entrapped in photo-crosslinked resin gels of a suitable net-work structure. Acetone-dried cells ofA. simplexhaving an induced steroidΔ1-dehydrogenase activity were also entrapped with photo-crosslinkable resin prepolymers and used forΔ- dehydrogenation of hydrocortisone to prednisolone. For the production of prednisolone from cortexolone, the combination of sequential steps, 11β-hydroxylation and subsequentΔ1-dehydrogenation, was found to be suitable. Each immobilized microbial cell system was stable and could be used for the sequential reactions repeatedly (operational period, 25
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