A new sensitive, specific radioimmunoassay for human alpha atrial natriuretic peptide (hANP) and a novel extraction method for hANP have been developed. Antiserum to hANP (Keq = 1.923 #xD7; 10111/mol) showed no cross-reactivity with related analogues. Displacement of 50 bound125I-hANP occurred at 4.7 #xB1; 0.1 fmol/tube (n=15). The limit of detection of plasma hANP after extraction of 2ml plasma with Florisil was 1.2 pmol hANP/litre plasma. The recovery of synthetic hANP from plasma over the range 6.5#x2013;162.5 pmol/1 was 71.2 #xB1; 1.9. Inter- and intra-assay coefficients of variation were 13.1 and 10.1 respectively. Extracted plasma was stored at #x2212;80#xB0;C without loss in immunoreactivity. The radioimmunoassay was physiologically validated in man by measuring plasma hANP following central volume expansion -
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