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>Insulin‐like growth factor 1, insulin, and progesterone induce early and late increases inXenopusoocytesn‐1, 2‐diacylglycerol levels before meiotic cell division
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Insulin‐like growth factor 1, insulin, and progesterone induce early and late increases inXenopusoocytesn‐1, 2‐diacylglycerol levels before meiotic cell division
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机译：Insulin‐like growth factor 1, insulin, and progesterone induce early and late increases inXenopusoocytesn‐1, 2‐diacylglycerol levels before meiotic cell division
AbstractAfter a 3 to 6 hour incubation, addition of progesterone (the most effective), insulin‐like growth factor 1 (IGF‐1; the second most effective), or insulin induces meiotic cell division inXenopusoocytes. Measurement of an endogenous activator of protein kinase C, sn‐1,2‐diacylglycerol (DAG), by an enzymatic method recording mass demonstrates that all three hormones alter DAG levels. Five seconds after addition, only progesterone transiently reduces DAG levels by about 25. At 15 minutes after addition, all three hormones produce a peak of DAG (115 to 160 of control values), with the more effective hormones producing a larger increase in DAG. Insulin produces the smallest DAG increase, but the DAG release is longer lasting. Finally, all three hormones induce a second peak in DAG levels just before white spot appearance (at 0.85 GVBD50, where 1.0 GVBD50is when 50 of the cells have divided). With these data and since an activator of protein kinase C, a phorbol ester, has been found to induce meiosis, the kinase may play a role in early proliferative events at the plasma membrane and in late events at the
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