Nine metastatic melanoma cell lines and two melanocyte cell lines were analyzed for point mutations in highly conserved regions of thep53gene. No mutations were detected in the two melanocytic cell lines and in eight melanoma cell lines. However, a C→T transition at codon 248, resulting in a substitution of tryptophan for arginine, was found in one melanoma cell line. On immunohistochemical staining, only this cell line showed reactivity for mouse monoclonal antibody 1801, which is immunoreactive with humanp53protein. The original paraffin‐embedded specimen from which this mutant cell line was established was obtained, and sequence analysis detected the identical mutation in thep53gene as that seen in the derived cell line. This is the first report indicating point mutations in thep53gene in malignant melanocytic tiss
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