AbstractProtein determinations of 172 grain and dhal (decorticated dry split seed) pigeonpea samples were carried out using three methods: (a) micro‐Kjeldahl (MKJ), (b) colorimetric estimation of NH4+ with phenol hypochlorite reagents using the Technicon Auto‐Analyser (TAA), and (c) dye‐binding capacity (DBC) method using Acid Orange 12 dye. Protein percentages determined by the TAA and MKJ methods were highly correlated for whole‐grain (0.948**) and dhal (0.967**) samples. The DBC method gave reliable results for dhal samples only. In the DBC procedure, higher protein percentages were recorded with smaller flour particles and longer mixing time, but different temperatures and durations of heating had no effect. Positive and highly significant correlations were obtained between the protein values of whole‐grain and dhal samples in all the methods. Small grains gave a lower correlation between whole‐grain and dhal protein content due to the observed negative correlation between grain size and percentage o
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