Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

ZAN HONG; MING YEH

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Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

机译：Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

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Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgE-producing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibodyresponses. Here we report the construction of a normal human IgE combinatorial library. The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.

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来源
《Cell research》 |1996年第1期|1-9|共9页
作者
ZAN HONG; MING YEH;
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作者单位

Shanghai Institute of Cell Biologty, Chinese Academy of Sciences, Shanghai 200031, China;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Phage antibody library; human IgE(Fab); human anti-trichosanthin IgE;

Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

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