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>The use of immobilized concanavalin a for the separation of alternansucrase from dextransucrase in culture broth ofLeuconostoc mesenteroidesNRRL B-1355
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The use of immobilized concanavalin a for the separation of alternansucrase from dextransucrase in culture broth ofLeuconostoc mesenteroidesNRRL B-1355
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机译:The use of immobilized concanavalin a for the separation of alternansucrase from dextransucrase in culture broth ofLeuconostoc mesenteroidesNRRL B-1355
Immobilized concanavalin A has been used to bind a polysaccharide-glucosyltransferase complex fromLeuconostoc mesenteroidesNRRL B-1355, which is capable of synthesizing the unusual D-glucan alternan from sucrose. The dextransucrase present in culture fluid passes through a column of immobilized concanavalin A without binding, while the portion of alternansucrase that does bind is eluted using 1-O-methyl α-D-mannopyranoside
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